Gene Technology and Plant Genetic Transformation Methods

Thirty years of plant transformation technology development.

Technology development is seminal to many aspects of basic and applied plant transgenic science. Through the development and commercialization of genetically modified crops, the evolution of plant transgenic technologies is also relevant to society as a whole. In this study, literature statistics were used to uncover trends in the development of these technologies. Publication volume and impact...

Genetic Methods in Plant Breeding

Definition of plant breeding, Sources of genetic variability Significance of genetic variability. Selection methods (SM). Factors affecting (SM). Heterosis & examples Hybridization crossing methods –inbreeding/ outcrossing. Convergent breeding, Divergent breeding. Evaluation of inbred lines by: Single crosses; 3-way crosses, Performing top crosses, Diallel analysis. Combining ability. General &...

Gene Targeting and Genetic Transformation of Plants

Genetic Transformation of Cells using Physical Methods

The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic ...

Methods for genetic transformation of filamentous fungi

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking ...